燃料与化工 ›› 2012, Vol. 43 ›› Issue (6): 35-38.

• 煤气净化与化学产品加工 • 上一篇    下一篇

对二甲苯降解菌的筛选及特性研究

魏 鑫 颜家保 王巧凤 游 海   

  1. 武汉科技大学化学工程与技术学院,武汉 430081
  • 出版日期:2012-11-30

Study on selection and features of  p-xylene degradation bacterial strain

Wei Xin    Yan Jiabao    Wang Qiaofeng    You Hai   

  1. School of Chemical Engineering and Technology,  Wuhan University of Science and Technology, Wuhan 430081,  China

  • Online:2012-11-30

摘要:

以对二甲苯为唯一碳源和能源,筛选得到一株对对二甲苯具有高效降解能力的菌株,生理生化实验、16S rDNA基因鉴定结果表明该菌株属于假单胞杆菌属,其最佳降解条件为pH=7.5,培养温度为30℃,摇床转速为150r/min。将高效菌接种至实际废水中,能在短时间内将废水中的对二甲苯全部降解。

Abstract:

By taking p-xylene as the only carbon source and energy, one bacterial strain which has a high efficiency degradation capability to p-xylene is obtained by selection. The results of physiological and biochemical experiment and 16S rDNA gene identification indicate that the bacterial strain belongs to pseudomonas sp. Its optimum degradation conditions are pH=7.5, 30 ℃ cultivation temperature and mechanical shaker’s speed of 150r/min. The high efficiency bacterial strain is put into actual waste water and can degrade all p-xylene in it in a short time.

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